Total Internal Reflection Fluorescence

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TIRF Slides  Lightguides and Prisms


TIRF and TIRF-EC lightguides. TIRF and TIRF-EC slides

Chemically modified and biologically functionalized TIRF and TIRF-EC lightguides,

and TIRF and TIRF-EC slides and coverslips

TIRF Labs offers untreated, chemically modified, and biologically functionalized TIRF and TIRF-EC slides, coverslips, and TIRF and TIRF-EC lightguides.

See below the definition of TIRF slides and TIRF lightguides


Definitions:

TIRF slide - optical substrate with two large surfaces polished and side facets ground or raw cut. Raw cut is a wavy surface obtained after breaking a glass plate scratched on the surface. If the dimensions of a rectangular slide are: 25.4 mm x 76.2 mm x 1.0 mm, only two large surfaces 25.4 mm x 76.2 mm are polished. End facets 25.4 mm x 1.0 mm and side facets 76.2 mm x 1.0 mm are ground or raw cut.  TIRF slides are designed for use with prism-based TIRF. TIRF slides can be used for other than TIRF applications, for example, epi-fluorescence experiments. TIRF slides are not intended for use with lightguide-based TIRF (lg-TIRF). Because end facets of TIRF slides are ground or raw cut; they exhibit compromised quality of coupling light through one of the end facets.


Float-polished vs. abrasive-polished slides and coverslips. Typically, low-cost slides and coverslips are float- or fusion-overflow-polished. The former technology is used by Schott and other firms, the latter - by Corning and others. The quality of their surfaces is sufficient for many applications, including certain TIRF experiments. However, for demanding applications it is good idea to use certified abrasive-polished slides and coverslips with the lowest “scratch and dig” numbers. For “scratch and dig” definitions see the URL: https://www.edmundoptics.com/resources/application-notes/optics/understanding-surface-quality-specifications/


TIRF-EC slide is a TIRF slide, one large surface of which is coated with optically transparent electroconducting layers of Indium Tin Oxide (ITO) or tin dioxide SnO2. TIRF-EC slides are designed for TIRF experiments in prism-based TIRF geometry with electrochemical and electric field control (TIRF-EC), including spectroelectrochemical studies, use of electrophoretic and dielectrophoretic effects in the TIRF flow chamber, or in a compartments adjacent to the TIRF flow chamber. More expensive TIRF-EC lightguides are not necessary for TIRFing with prism-based geometry, but can be used instead of TIRF-EC slides.


TIRF lightguide is a TIRF slide with one or two end facets polished. If the dimensions of a rectangular lightguide are: 25.4 mm x 76.2 mm x 1.0 mm, two large surfaces 25.4 mm x 76.2 mm, and at least one of the end facets 25.4 mm x 1.0 mm of the lightguide are polished. Polishing of end facets allows for minimizing scatter of excitation light and facilitates coupling of excitation light into the lightguide. After entering through one of the end facets, propagating excitation light, similar to reflections in an optical fiber, undergoes multiple reflections in TIRF lightguide from the top and the bottom of the lightguide that are large surfaces 25.4 mm x 76.2 mm in the above case. In the case of 0.17-mm-thick lightguide the reflections occur between large surfaces that are 0.17 mm apart.


TIRF-EC lightguide is a TIRF lightguide, one large surface of which is coated with optically transparent electroconducting layers of Indium Tin Oxide (ITO) or tin dioxide SnO2. In other words, TIRF-EC lightguide is a TIRF-EC slide, at least one end facet of which is optically polished to minimize scatter of excitation light entering through the end facet. TIRF-EC lightguides are designed for TIRF experiments with lightguide-based TIRF geometry with electrochemical and electric field control (TIRF-EC), including electrophoresis and dielectrophoretic effects in the TIRF flow chamber, or in a compartments adjacent to the TIRF flow chamber. We do not recommend using TIRF-EC slides instead of TIRF-EC lightguides, because the former produce additional scatter at the interface, where excitation light enters the lightguide.


       TIRF slides and TIRF lightguides are made of selected low fluorescence glass, silica, and  sapphire to minimize the background fluorescence and scatter. Refractive indices of glass coverslips and slides are 1.52-1.53 @ 546 nm; silica – 1.46 @546 nm,   sapphire – 1.76-1.77 @ 532 nm. Sapphire coverslips are made of crystalline synthetic sapphire with random orientation. Sapphire crystals exhibit small birefringence with refractive indices for the extraordinary and ordinary beams 1.76486 and 1.7717, respectively [http://www.tydexoptics.com]. Typically, for TIRF and TIRF-EC applications small birefringence of a 170-micron sapphire coverslip is not noticeable. Typical sapphire coverslip contains traces of chromium, which results in red auto-fluorescence.

TIRF and TIRF-EC slides. TIRF and TIRF-EC lightguides

Untreated, chemically modified, and biologically functionalized TIRF and TIRF-EC slides, lightguides, and coverslips

TIRF and TIRF-EC slides

TIRF and TIRF-EC lightguides

Chemically modified and biologically functionalized

TIRF and TIRF-EC lightguides, and TIRF and TIRF-EC slides and coverslips

 Product number
TIRF and TIRF-EC slides, and TIRF and TIRF-EC lightguides
Untreated
Price USD per one slide
U1
Al2O3_2240170

Sapphire TIRF lightguides 22 x 40 x 0.17 mm. Two large surfaces 22 x 40 mm and one end facet 22 x 0.17mm are polished. Minimum order - 5 slides.
$125.00
U2
Al2O3_2040170

Sapphire TIRF lightguides 20 x 40 x 0.17 mm. Two large surfaces 20 x 40mm and one end facet 20 x 0.17mm are polished. Minimum order - 5 slides.
$125.00
U3
LG2240170

Low fluorescence glass lightguides 22x40x0.17 mm. Minimum order - 5 slides.
$50.00
U4
LSiO225751000

Silica 1-mm TIRF lightguides 25.4x76.2x1.0mm. Two large surfaces 25x76mm and both end facets 25x1mm are polished. Minimum order - 5 slides.
$125.00
U5
SG25751000-ITO

ITO-coated 1-mm glass TIRF-EC slides ~25x76x1mm. One large surface 25x76mm is coated with optically transparent layer of Indium Tin Oxide (ITO). Minimum order - 5 slides.
$220.00
U6
LSG25751000-ITO

ITO-coated 1-mm glass TIRF-EC lightguides ~25x76x1mm. Two large surfaces 25x76mm and both end facets 25x1mm are polished. One large surface 25x76mm is coated with optically transparent layer of Indium Tin Oxide (ITO).  Minimum order - 5 slides.
$186.00
U7
SiO225381000

Silica 1-mm TIRF slides 25x38x1.00 mm. Two large surfaces 25x38mm are polished. Minimum order - 5 slides.
$69.00
U8
SiO225381000-ITO

ITO-coated 1-mm silica TIRF-EC slides 25x38x1.00 mm. One large surface 25x38mm is coated with optically transparent layer of Indium Tin Oxide (ITO).Minimum order - 5 slides.
$160.00
Product number
Chemically Modified  TIRF and TIRF-EC slides and lightguides
Price USD per one slide
C1

Al2O3_2040170-OH

Superclean highly hydrophilic sapphire lightguides 20x40x0.17mm. Minimum order - 5 slides.
TBD
C2
Al2O3_2040170-CH

 Hydrophobic sapphire lightguides 20x40x0.17 mm. Minimum order - 5 slides.
TBD
C3
Al2O3_2040170-Am

Aminated sapphire lightguides 20x40x0.17 mm. Minimum order - 5 slides.
TBD
C4
Al2O3_2040170-Ald
Aldehyded sapphire lightguides 20x40x0.17 mm. Minimum order - 5 slides.
TBD
C5
Al2O3_2040170-Epx
Epoxy-modified sapphire lightguides 20x40x0.17 mm. Minimum order - 5 slides.
TBD
C6
LG2240170-OH
Superclean highly hydrophilic low fluorescence glass lightguides  22x40x0.17 mm. Minimum order - 5 slides.
TBD
C7
LG2240170-CH
Hydrophobic  low fluorescence glass lightguides 22x40x0.17 mm. Minimum order - 5 slides.
TBD
C8
LG2240170-Am
Aminated low fluorescence glass lightguides 22x40x0.17 mm. Minimum order - 5 slides.
TBD
C9
LG2240170-Ald

 Aldehyded low fluorescence glass lightguides 22x40x0.17 mm.  Minimum order - 5 slides.
TBD
C10
LG2240170-Epx

Epoxy-modified low fluorescence glass lightguides 22x40x0.17mm.  Minimum order - 5 slides.
TBD
C11
SiO225751000-OH

Superclean highly hydrophilic low fluorescence silica 1-mm TIRF lightguides 25x76x1mm.  Minimum order - 5 slides.
TBD
C12
SiO225751000-CH

Hydrophobic  low fluorescence silica 1-mm TIRF lightguides 25x76x1mm.  Minimum order - 5 slides..
TBD
C13
SiO225751000-Am

Aminated low fluorescence silica 1-mm TIRF lightguides 25 x 76 x 1 mm.  Minimum order - 5 slides.
TBD
C14
SiO225751000-Ald

 Aldehyded low fluorescence silica 1-mm TIRF lightguides 25 x 76 x 1 mm.  Minimum order - 5 slides.  
TBD
C15
SiO225751000-Epx

Epoxy-modified low fluorescence silica 1-mm TIRF lightguides 25x76x1mm.  Minimum order - 5 slides.
TBD
Product number
Biologically Functionalized TIRF and TIRF-EC slides and lightguides
Price USD
B1.
Al2O3_2040170-Btn

  Biotinylated sapphire lightguides 20x40x0.17 mm. Minimum order - 5 slides.
TBD
B2.
Al2O3_2040170-Stv
Streptavidin-coated sapphire lightguides 20x40x0.17 mm. Minimum order - 5 slides.
TBD
B3.
LG2240170-Btn
Biotinylated low fluorescence glass lightguides 22x40x0.17 mm.  Minimum order - 5 slides.
TBD
B4.
LG2240170-Stv
Streptavidin-coated low fluorescence glass lightguides 22x40x0.17 mm.  Minimum order - 5 slides.
TBD
B5.
SiO225751000-Btn
Biotinylated low fluorescence silica 1-mm TIRF lightguides 25x76x1mm.  Minimum order - 5 slides.
TBD
B6.
SiO225751000-Stv
Streptavidin-coated low fluorescence silica 1-mm TIRF lightguides 25x76x1mm.  Minimum order - 5 slides.
TBD

TIRF Prisms

dovetail TIRF prisms

semisphere TIRF prisms

TIRF Prisms:

We carry in stock over 20 types of dovetail and semisphere TIRF prisms and can manufacture a custom-design prism according to your specifications. Request additional info via email:

info@tirf-labs.com