Single Molecule Detection, sm-FRET, Single Cell Analysis, Cell Membrane Studies, TIRF microscopy, TIRF accessories for fluorometers, TIRF biosensor instruments, TIRF electrochemistry, TIRF dielectrophoresis, Real-time microarrays, iDiagnostics - cellphone based TIRF, and more>>>
Total Internal Reflection Fluorescence (TIRF)
Single Molecule Detection, including sm-FRET,
Plasma Membrane Studies, Single Cell Analysis, Real-time Microarrays, ...
TIRF Principles. TIRF employs the phenomena of total internal reflection and the evanescent wave to selectively excite fluorophores located at the surface and in close proximity (~100 nm) to the surface of a glass, silica, or plastic slide. Fluorophores that are in the bulk of solution are not excited and do not fluoresce. Exceptional spatial selectivity enables TIRF with numerous advantages, including the ultimate limit of detection - single molecule. TIRF efficiently minimizes the background and allows for the real-time monitoring the molecular dynamics at the level of single molecules. More »
TIRF Applications. TIRF spectroscopy and TIRF microscopy are powerful analytical tools for numerous life science areas, including the analysis of biomolecular interactions, single molecule detection, cell biology, real-time microarrays for molecular diagnostics, bioassay development, nanoengineering, and drug screening. In particular, TIRF is “a method uniquely suited to image the plasma membrane with its associated organelles and macromolecules in living cells. The method shows even the smallest vesicles made by cells, and can image the dynamics of single protein molecules.” [Steyer JA, Almers W. A real-time view of life within 100 nm of the plasma membrane. Nature Reviews, Molecular Cell Biology, 2001, 2(4), 268-75]. References to thearticles published by our customers and TIRF application notes are available at www.tirf-labs.com/applications.html More »
TIRF Products. TIRF Labs offers a broad range of TIRF products: prism-, lightguide-, and objective-based TIRF microscopy, from simple add-on accessories - to sophisticated computer-controlled turnkey stations. Our LED and laser fluorescence illuminators include cost-efficient single-color light sources, two-, three-, four-color, and seven-color computer-controlled illuminators. For TIRF spectroscopy we supply TIRF accessories with gravity flow and digital fluidics. Our automated TIRF microarray biosensors detect multiple nucleic acid, protein, and metabolite markers in parallel and in a matter of a few minutes. Popular products also include TIRF slides, lightguides, and prisms. More »
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TIRF Geometries. Prism-, lightguide-, and objective-based optical schemes are three popular geometries that are used for TIRF microscopy. Each geometry has its own set of advantages and limitations. A Prism-based scheme provides the best signal-to-background ratio, but is difficult to implement with an open perfusion chamber on an inverted microscope. A lightguide-based geometry yields exceptional flexibility and superior signal-to-noise ratio, can be used with open perfusion chamber on inverted microscopes, is compatible with dry, water-, and oil-immersion objectives, but requires larger optical power to obtain equal intensity of the evanescent wave. An objective-based scheme collects the largest portion of the emitted fluorescence, but the quality of TIRF effect is severely compromised, because the excitation light shares the same optics with the emission channel; large intensity of the stray light excites the bulk of the specimen and deteriorates the TIRF effect produced by objective-TIRF. TIRF Microscopy webpages will help you to choose a geometry suited for your TIRF applications. TIRF Products webpages will help to select illuminators, fluidics, electrochemical and dielectrophoretic control, other devices and supplies that are necessary for TIRFing. TIRF Labs offers the entire range of TIRF instrumentation: from inexpensive add-on TIRF accessories for microscopes and fluorometers -to turnkey TIRF microscopy stations and automated TIRF biosensor instruments. More »
Visit also: www.TIRFmicroscopy.com
TIRF microscopy re-invented:
TIRF Labs is now offering for sale advanced lightguide- and prism-based TIRF systems that feature optical schemes with the excitation path naturally independent from the emission channel. Unlike in objective-type TIRF, the intensity of stray light in lg- and p-TIRF is minimal and the TIRF effect is clean.
LgTIRF and pTIRF systems can be used with dry, water-, and oil-immersion objectives.