Applications of Total Internal Reflection Fluorescence (TIRF)

TIRF Applications include:

  • Single molecule detection, including smFRET
  • Super resolution microscopies
  • Cell membrane studies
  • Molecular diagnostics
  • Analysis of biomolecular interactions
  • Monitoring real-time kinetics of biomolecular interactions
  • Real-time TIRF microarrays
  • Combined DNA, RNA, protein, and metabolite arrays
  • Studies of protein-protein and protein-DNA interactions
  • Studies of surface supported membranes
  • Dynamics of lipid rafts studies
  • Nanoengineering
  • Bioassay development, and more …

TIRF Microscopy Application Notes

Application note brochures and PDF reprints of selected articles published by our customers, who used our pTIRF and lgTIRF systems as accessories for fluorescence microscopes.

TIRF Spectroscopy Application Notes

PDF reprints of selected articles published by our customers, who used our TIRF flow system TA1004 as accessory for fluorometers.

  Selected articles with data obtained by TIRF Labs’ pTIRF and lgTIRF microscopy systems and TIRF accessories for fluorometers
  Lightguide-based Total Internal Reflection Fluorescence and Shallow Angle Fluorescence Microscopy (SAFM) – Novel Methods for Cell Biology Studies
  lg-TIRFM for Neuroscience Studies. Axon Growth and Pathfinding. Studies of Intracellular Pathways that Govern Axon Behavior
  Prism-based TIRFM and lightguide-based TIRFM for Single Molecule Detection Studies
  TIRF-EC – the Utility of ElectroChemical Polarization and Electric Field Control for TIRF Detection
  Molecualr mechanisms of baculovirus entry into mamallian cells studied by lgTIRFM, and SAFM combined with other methods
  TIRF Spectroscopy Application Note 1A Label-Free Continuous Total-Internal-Fluorescence-Based Immunosensor
  TIRF Spectroscopy Application Note 2 Displacement of Adsorbed Insulin by Tween 80 Monitored Using Total Internal Reflection Fluorescence and Ellipsometry
  TIRF Spectroscopy Application Note 3 Enzymatic lipid removal from surfaces—lipid desorption by a pH-induced “electrostatic explosion”
  TIRF Spectroscopy Application Note 4 Regenerable Biosensor Platform: A Total Internal Reflection Fluorescence Cell with Electrochemical Control
  TIRF Spectroscopy Application Note 5 Adsorption of Poly(ethylene glycol)-Modified Lysozyme to Silica
  TIRF Spectroscopy Application Note 6 Adsorption of plasma proteins on polyethylene oxide-modified lipid bilayers studied by total internal reflection fluorescence